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We concatenated them maintaining the same order for each haloarchaeon to form sequences ready to be aligned with the Clustal W (Larkin et al., 2007) plugin inside Geneious 7.1 software using the IUB DNA weight matrix. The resulted alignment was used for construction of the maximum likelihood phylogenetic tree using PhyML program version 3.0 (Guindon and Gascuel, 2003) with the Tamura Nei substitution model.
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